蝉拟青霉多糖的提取纯化工艺研究

Studies of The Opbimum Exbruebim and Depunotion Praedure for Polysaccharide from Cordyceps Cicadae

目的:确定蝉拟青霉多糖的最佳提取纯化工艺。方法:用苯酚—硫酸紫外分光光度法测定蝉拟青霉多糖含量,采用正交实验设计,以多糖含量为指标,优化蝉拟青霉多糖水提取和醇沉淀工艺。结果:蝉拟青霉多糖水提取最佳条件:加水量1:7,提取时间1h,提取次数3次;醇沉淀最佳条件:水提液浓度0.15g生药/mL,醇浓度60%,醇沉时间72h。结论:优化的蝉拟青霉多糖水提醇沉工艺简便、合理、可行。

Objective： To select the optimum extraction and depuration procedure for polysaccharide from Cordyceps cicadae. Methods ： UV - Spectrophotometry was selected to determine the content of polysaccharide. By orthogonal test design, to select the optimum procedure of extraction with water and precipitation with alcohol. Results： The optimum extraction procedure was medical material decocted lh with 7 times of water and 3 times and the optimum depuration procedure was the solution was concentrated to 0. 15g Cordyceps cicadae per milliliter and precipitated 72h with 60% alcohol. Conclusion： The optimum extraction and depuration procedure was simple,resonable and reliable.