花生晚斑病抗性AFLP标记

Identification of AFLP markers linked to resistance to late leaf spot in peanut(Arachis hypogaea L.)

以抗感晚斑病组合"中花5号×ICGV 86699"的F2分离群体为材料,经田间抗性鉴定明确抗性亲本ICGV 86699的抗性受隐性单基因或主效基因控制;AFLP分析结合BSA法筛选到与晚斑病抗性连锁较紧密的AFLP标记3个,即E35/M51、E37/M48和E41/M47,它们与抗性间的图距分别为7.40cM、7.40cM和8.67cM;所获得的3个标记间连锁紧密,位于同一连锁群上。这3个标记在具有野生种亲缘的7个抗病花生品种或材料中均能检测到,而在栽培种以多粒型为代表的抗病材料中均未检测到,表明ICGV 86699的抗性基因与栽培种花生中的抗性基因不同。这是国内外有关花生晚斑病抗性分子标记的首例报道。

Late leaf spot （LLS） cause substantial yield losses and reduce seed quality in peanut （Arachis hypogaea L. ）. Adoption of resistant cultivars is the best option to overcome yield losses. A line highly resistant to LLS derived from an interspecific hybridization, ICGV 86699, was used to identify AFLP markers for LLS resistance. The resistance to LLS in ICGV 86699 was found to be controlled by a single recessive gene based on the resistance identification and analysis of segregation in the F2 population of Zhonghua No. 5 × ICGV 86699. Amplified fragment length polymorphism （AFLP） analysis and bulked segregant analysis （BSA） were employed to identify DNA markers linked to LLS resistance based on the segregating F2 population of the cross. Three AFLP markers, E35/M51, E37/M48 and E41/M47, linked to the LLS-resistant gene were identified. The three markers were also linked each other on same linkage group. The map distance between the markers and the resistant gene were 7. 40, 7.40, and 8. 67 cM, respectively. The three markers were detected within 7 resistant interspecific hybrid lines, but weren＇t detected within 10 cultivated peanut with resistance and 13 susceptible lines. This is the first report on the identification of molecular markers closely linked to LLS resistance in peanut.