重叠HCV感染对HBV/C基因热点变异的影响

Influence of hepatitis C virus surperinfection upon core “hot” mutations of hepatitis B virus

为探讨乙型肝炎病毒 (HBV)重叠丙型肝炎病毒 (HCV)感染时 HCV对 HBV复制和基因变异的影响 ,采用套式聚合酶链反应 (PCR)与限制性片段长度多态性 (RFLP)相结合 ,对 1 9例 HBV感染重叠 HCV感染 (A组 )和 31例单独 HBV感染 (B组 )的慢性肝病患者分析前 C区密码 2 8终止变异(A83)和 C区密码 97异亮氨酸变为亮氨酸变异 (L97)。结果显示 A组第一次 PCR阳性率 (1 6% )明显低于 B组 (65% ) (P<0 .0 0 1 ) ,A组 L97和 A83检出率分别为 35%和 2 8% ,明显低于 B组的 57%和 68%(P<0 .0 0 1 )。结果提示 :慢性乙型肝炎重叠 HCV感染 ,HCV对 HBV复制有抑制作用 ,HBV发生前

To study the influence of hepatitis C virus (HCV) superinfection upon the replication and mutation of hepatitis B virus (HBV). Group A were 19 cases with superinfection of HBV and HCV, and group B were 31 cases with HBV infection alone, of which the HBV precore/core regions were amplified with nested polymerase chain reaction (PCR), and the precore region codon 28 stop mutation (A83) and the core region mutation of codon 97 leucine substitute for isoleucine (L97) were analysed by restriction fragment length polymorphism (RFLP). Data showed that the positive rate of 1st PCR was significantly lower in group A (16%) than in group B (65%) (P<0.001) as well as the positive rates of L97 and A83 in group A (35% and 28%, respectively) were significantly lower than that in group B (57% and 68% respectively, P<0.001).The results suggest that the superinfection of HCV inhibited the replication of HBV and decreased the frequency of precore/core mutation in HB patients with superinfection of HCV.