TNF-〆、IL-6和IL-8与慢性肝病的关系

Relationship between TNF-〆、 IL-6、 IL-8 and chronic liver disease

为研究肿瘤坏死因子 -α( TNF-α)、白细胞介素 - 6( IL - 6)和白细胞介素 - 8( IL - 8)与慢性肝病的关系 ,分别用 RIA和 EL ISA法检测 94例慢性肝病患者血清 TNF-α和 IL - 8含量 ,同时用免疫组化 ABC法观察其中 2 5例患者 (慢迁肝 3例 ,慢活肝 5例 ,肝硬变 17例 )肝组织内 TNF-α,IL - 8和 IL - 6的表达和分布。结果显示 :慢活肝和肝硬变患者血清及肝组织内上述细胞因子水平均明显增加 ( P<0 .0 5～0 .0 1) ,且分别与血清中胆红素水平和肝组织炎症活动指数密切相关 ( P<0 .0 5～ 0 .0 1)。提示这些细胞因子可能相互影响 ,并在慢性肝脏炎症损伤。

To study the relationship between tumor necrosis factor α (TNF α), interleukin 8(IL 8), interleukin 6(IL 6) and chronic liver disease, radioimmunoassay (RIA) and enzyme liked immunosorbent assay (ELISA) were performed in 94 patient with chronic liver disease to determine the serum levels of TNF α and IL 8, whereas the intrahepatic TNF α、 IL 8 and IL 6 were determind by immunohistochemical methods (ABC) for liver tissue sections from 25 patients, including 3 patients with chronic persistent hepatitis, 5 patients with chronic active hepatitis and 17 patients with liver cirrhosis. Results showed that TNF α、 IL 8 and IL 6 levels in both serum and liver tissue sections of CAH and LC patients increased significantly as compared with normal subjects (P<0 05～0 01), moreover, the contents of these cytokines correlated closely with the serum levels of BIL and histologic inflammatory activity index (HAI) (P<0 05～0 01). The results indicated that TNF α, IL 6 and IL 8 maybe affected each other and play an important role in process of inflammatory injury, repair and fibrosis in chronic liver disease.