补骨脂素对MCF-7/ADR耐药细胞内ADR及Ca^(2+)浓度的影响(英文)

Effects of Psoralen on ADR Multidrug Resistance and Ca^(2+)Concentration in MCF-7/ADR Cells

目的研究补骨脂素对MCF-7/ADR耐药细胞逆转作用及对耐药细胞内Ca2 +浓度影响,探讨其可能作用机制。方法采用MTT法测定补骨脂素对细胞增殖抑制作用,高效液相色谱法检测细胞内ADR的浓度,激光共聚焦显微镜测定细胞内不同时段的Ca2 +浓度。结果补骨脂素在1 ~20μmol/L浓度下能不同程度降低ADR对MCF-7/ADR细胞的IC50,并不同程度提高细胞内ADR浓度;1 ~20μmol/L补骨脂素在不同作用时段(24、48、96h)对MCF-7/ADR细胞内Ca2 +浓度与作用时间呈负相关。结论补骨脂素能逆转MCF-7/ADR细胞的MDR,其作用机制与影响耐药细胞内Ca2 +浓度,故而增加细胞内ADR浓度有关。

Objective To research the reversal effects of psoralen on multidrug resistant action and its influence on intracellular Ca^2＋ concentration in MCF-7/ADR cells and to explore its possible mechanisms of reversing multidrug resistance （MDR）. Methods The inhibitory effects of psoralen on the viability of MCF-7/ADR cells were determined with MTT assay, intracellular adriamycin （ADR） concentration was assayed with HPLC and the intracellular Ca^2＋ concentrations in different incubative duration were detected with confocal microscope. Results Psoralen from 1 to 20 μmol/L reduced the value of IC50 of ADR in MCF-7/ADR cells, enhanced accumulation of ADR and influenced Ca^2＋ concentration with a negative correlation in different duration （24 h, 48 h and 96 h）. Conclusion Psoralen can reverse MDR in MCF-7/ADR cells and its mechanism may be related to the increase of the intracellular accumulation of ADR by enhancing the intracellular Ca^2＋ concentration.