人骨肉瘤鸡胚移植瘤模型的生物学特性研究

Establishment of a human osteosarcoma model on the chick embryo chorioallantoic membrane and its biological properties

目的:建立人骨肉瘤鸡胚移植瘤模型,研究其形态学及生物学特性。方法:将人骨肉瘤细胞接种于鸡胚绒毛尿囊膜(chick embryo chorioallantoic membrane,CAM),观察影响骨肉瘤鸡胚移植瘤成活的因素、移植瘤生长特性、形态学特征和生物学性状。结果:建立了人骨肉瘤鸡胚移植瘤模型。移植瘤易于生长,具有较强的血管诱导作用。光镜下移植瘤具有与人骨肉瘤类似的组织结构。结论:该模型易于复制,能动态观察骨肉瘤诱导的血管生成过程,可用于骨肉瘤的生物学行为研究、药物筛选等领域。

To establish a human osteosarcoma model on the chick embryo chorioallantoic membrane （CAM） and study its morphological and biological properties. Methods： Human osteosarcoma cell line HOS -8603 was cultivated and implanted on the CAMs. The factors affecting the implanted tumors and the morphological and biological properties of the tumors were studied. Results： A human osteosarcoma model on CAM was established . The implanted tumors were easy to grow on the CAMs and had a strong trend of angiogenesis and similar structure as human osteosarcoma with microscopic verification. Conclusion： This model is of easy duplication and simple operation, and can be used in the research of the biological properties and drug selection for human osteosarcoma.