肺腺癌肿瘤相关巨噬细胞的表达及与肿瘤血管生成的关系

Study of tumor-associated macrophage expression and angiogenesis in pulmonary adenocarcinoma

目的:研究肺腺癌(Pulmonary adenocarcinoma)组织中肿瘤相关巨噬细胞(tumor-associated macro-phages,TAMs)的表达与临床病理学特征的关系及对肿瘤血管生成的影响。方法:应用免疫组化S-P法检测49例肺腺癌组织中TAMs、微血管密度(microvessel density,MVD)、血管内皮细胞因子(vascular endothelialgrowth factor,VEGF)的分布,并用计算机图像分析系统进行分析。结果:TAMs定量与淋巴结转移密切相关(P<0.01);与TNM分期有显著相关性(P<0.05)。肿瘤组织中MVD及VEGF表达高于对照组(P<0.05)。TAMs分布与MVD及VEGF呈正相关(r=0.56,P<0.05;r=0.58,P<0.05),VEGF表达与MVD呈显著正相关(r=0.334,P<0.01)。结论:TAMs表达可能与肿瘤血管生成有关,且影响肿瘤的生物学行为。

To investigate the expression of tumor - associated macrophage in pulmonary adenocarcinoma and the correlation between TAMs expression in pulmaonary adenocarcinoma and clinicopathological characteristics. Methods： The expression of tumor-associated macrophages （TAMs）, microvessel density（MVD） and vascular endothelial growth factor（VEGF） in tissues of 49 patients with pulmonary adenocarcinoma were detected by immunohistochemisty SP method and automated image analysis quantification. Results ： The quantitation of TAMs was strongly correlated with lymph node metastasis（ P 〈 0.01 ）, and it was significantly associated with TNM stage（ P 〈 0.05）. The mean MVD and VEGF counts in tumors was significantly higer than the counts in normal lung tissues. The expression of TAMs and MVD in pulmonary adenocarcinoma were significantly positively correlated （ r = 0.56, P 〈 0.05）, so were the expression of TAMs and VEGF（r = 0.58, P 〈 0.05）, and the expressions of VEGF and MVD（ r = 0.334, P 〈 0.01 ）. Conclusion： Tumor associated macrophages may play a major role in the regulation of angiogenesis in pulmonary adenocarcinoma and the biological behaviour of tumor.