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11β-羟类固醇脱氢酶在人肠癌中的表达 被引量：1

Expression of 11beta-hydroxysteroid dehydrogenase types 1 and 2 in colorectal cancer
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摘要 目的:观察11β-羟类固醇脱氢酶(11β-HSD)在肠癌中的表达。方法:12例确诊肠腺癌患者,术后立即取肿瘤组织和癌旁正常组织标本,采用半定量RT-PCR、Western-blotting等技术,以自身配对,研究11β-类固醇脱氢酶在肠癌中表达。结果:11β-羟类固醇脱氢酶(11β-HSD)两种同工酶在肠腺癌中表达不相同,在肿瘤组织中11β-HSD2表达明显减少,11β-HSD1在肿瘤与正常组织中表达无明显差异。结论:11β-HSD2可能与肠癌变有关。 To observe the expression of 11 beta - hydroxysteroid dehydrogenase types 1 and 2 in colorectal cancer. Methods： Neoplastic and autologous nonneoplastic tissue were collected from 12 patients with colorectal cancer. The mRNA expression ol 1 J3 - HSD1 and 11 J3 - HSD2 was detected by reverse transcription polymerase chain reaction （RT- PCR） . The protein expression of 11β- HSD1 and 11β- HSIY2 were detected by Western- blot assay. Results： Both isoforms of 11 J3 -HSD expressed in the colon adenocarcinoma, but their expression was not identical in neoplastic and non - neoplastic tissue. There was a significant decrease of 11β-HSD2 mRNA abun- dance and protein abundance in neoplastic tissue. 1118 - HSD1 protein and mRNA abundance was not significantly different from that in control tissue. Conclusion： Neoplastic transformati on is associated with decreasing of 11 11β- HSD2 mRNA and protein.
作者 王继荣 许红霞 陈刚 张鹏 鲁一兵 缪珩
机构地区 南京医科大学第二附属医院 江苏省肿瘤医院
出处 《现代肿瘤医学》 CAS 2007年第11期1619-1622,共4页 Journal of Modern Oncology
基金 南京医科大学科技发展基金重点项目(06NMUZ019)资助
关键词 肠癌 11β-羟类固醇脱氢酶I型基因 11β-羟类固醇脱氢酶2型基因 colorectal cancer human 11β-HSD1 gene human 11β-HSD2 gene
分类号 R735.3 [医药卫生—肿瘤]
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参考文献13

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同被引文献11

  • 1邵喜英,陈占红,曹江,方永明,王晓稼.pcDNA3.1(+)-CYP19-GFP真核表达质粒构建[J].浙江大学学报（医学版）,2011,40(2):189-194. 被引量：5
  • 2程庆丰,李启富.11β-羟类固醇脱氢酶1与代谢综合征关系研究进展[J].国外医学（内科学分册）,2005,32(12):525-529. 被引量：3
  • 3Campino C,Quinteros H,Owen G I,et al. 11β-hydroxysteroid dehydrogenase type 2 polymorphisms and activity in a Chilean essential hypertensive and normotensive cohort[J]. Am J Hypertens, 2012,25(5):597-603.
  • 4Dujic T T,Bego T,Mlinar B,et al. Association between 11beta-hydroxysteroid dehydrogenase type 1 gene polymorphisms and metabolic syndrome in Bosnian population[J]. Biochem Med(Zagreb), 2012,22(1):76-85.
  • 5Masuzaki H,Paterson J,Shinayama H,et al. A transgenic model of visceral obesity and the metabolic syndrome[J].Science, 2001,294(5549):2166-2170.
  • 6Paterson J M,Morton N M,Fievet C,et al. Metabolic syndrome without obesity: Hepatic overexpression of 11β-hydroxysteroid dehydrogenase type 1 in transgenic mice[J]. Proceedings of the National Academy of Sciences of the United States of America,2004,101(18):7088-7093.
  • 7Pereira C D,Azevedo I,Monteiro R,et al. 11β-hydroxysteroid dehydrogenase type 1: Relevance of its modulation in the pathophysiology of obesity, the metabolic syndrome and type 2 diabetes mellitus[J]. Diabetes Obes Metab,2012,14(10):869-881.
  • 8Turban S,Liu X,Ramage L,et al. Optimal elevation of beta-cell 11beta-hydroxysteroid dehydrogenase type 1 is a compensatory mechanism that prevents high-fat diet-induced beta-cell failure[J]. Diabetes,2012,61(3):642-652.
  • 9Xia G,Liu L,Xue M,et al.Discovery of novel sulfonamides as potent and selective inhibitors against human and mouse 11beta-hydroxysteroid dehydrogenase type 1[J]. Mol Cell Endocrinol,2012,358(1): 46-52.
  • 10万小莉,任伟.11β-羟基类固醇脱氢酶1与2型糖尿病[J].重庆医学,2007,36(19):2016-2018. 被引量：4

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现代肿瘤医学
2007年 第11期

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