鱼鳞中糖缀合物的提取与体外抗氧化活性研究

Research on the Isolation of a Glycoconjugate from Fish Scale and Its Antioxidant Activity

本文探索了利用碱浸提和酶法从淡水鱼鳞中提取糖缀合物的技术,通过考察NaOH浓度、料液比、提取时间对糖缀合物提取效率的影响,确定了其提取的最佳条件为:0.3 mol/L NaOH溶液、料液比为1:5、室温提取18 h。对提取物初步分析后,进行体外抗氧化活性研究,结果表明鱼鳞糖缀合物对DPPH.、O2.-、.OH有较强的清除能力,同时也有较好的还原能力和抗脂质过氧化能力。

The alkaline and enzymatic extractions of a glycoconjugate from fish scale were researched. The best extraction reagent, the concentration of the extraction reagent, the solid- fluid ratio and the extracting time were found to be NaOH solution, 0.3 mol/L, 1：5 and 18 h, respectively. Evaluation of the antioxidatnt activity of the achieved glycoconjugate showed that the glycoconjugate could efficiently scavenge DPPH, 02 and OH. Besides, it had high reducing capacity and strong ability to prevent lipid peroxidation.