激光扫描共聚焦显微镜图像的计算机处理

Computer processing and visualization of confocal microscopy images

为了研究激光扫描共聚焦显微镜图像的计算机处理,首先对图像进行预处理,包括光线吸收和散射的补偿以及基于最大后验盲解卷积,以提高图像质量。然后采用光线投射算法重建三维结构,实现三维图像平移、旋转、缩放、光线设置等操作。实验结果表明,该方法能够应用于普通配置计算机,可以处理各大品牌激光扫描共聚焦显微镜拍摄的图像序列,具有较快的处理速度和良好的人机交互等优点。

The methodology was proposed for image processing and interactive visualization of confocal microscopy images. Firstly,an intensity compensation algorithm was applied to reduce noises resulted from light absorption and scattering by objects and particles in the volume through which light passes. Secondly, a deconvolution algorithm based on maximum a posteriori was applied to improve the image stacks quality. At last, the ray-casting algorithm was used to reconstruct the fine details in the volume. Furthermore, virtual reality technique was integrated with volume rendering in order to provide users an intuitive interface to view the cells and its structures through translation, rotation and zoom operations. The speed and versatility of the program allows for convenient, fast, interactive examination of unknown cell structures even on common PC. The program can be applied for different types of confocal microscopy data sets with good results.