柴油降解菌的筛选及其降解特性

Bacteria Screening to Biodegrade Diesel and Their Characteristics

以市售0#柴油为惟一碳源对菌种进行筛选,得到2株高效降解柴油菌种Y1和Y2.经形态及生理生化特征分析,初步鉴定Y1为芽孢杆菌属(Bacillus),Y2为黄杆菌属(Flavobacterium).并对其生长曲线进行测定,为菌种的固定化提供了一定依据,以进一步对两株菌降解特性进行研究.结果表明:初始油质量浓度为150 mg/L、菌种Y1和Y2接种量为10%的条件下,经过48 h批培养实验,Y1和Y2的除油率分别为79.25%和77.23%,并随初始油质量浓度的增加而降低;同时观察到pH值显著影响两株菌的生理性质.

The commercial 0^# diesel was used as the only .source of carbon to screen out bacteria for diesel biodegradation and two strains were identified, i. e., Y1 Bacillus and Y2 Flavobacterium. Both were selected according to their morphological, physiological and biochemical characters. Then, their growing curves were plotted through tests to offer certain evidences for the immobilization of microorganism species and further study on the characters of biodegradation of the two bacteria strains in diesel. The results showed that when the initial oil concentration is 150 mg/L, and the strain Y1 or Y2 after batch culturing for 48 h has been inoculated into the diesel in a ratio of 1/10, the oil removal rate of Y1 and Y2 is 79.5% and 77.23%, respectively, and the effects of oil biodegradation of both Y1 and Y2 decrease with increasing initial oil concentration. It was also observed that the pH value affects greatly the physiological characteristics of Y1 and Y2.