多氯代甲烷对鳝鱼红细胞微核率及核异常率的影响

Effect of Polychlorinated Methane on the Micronucleus Rate and Nucleus Abnormity Rate of Mud Eel Red Blood Cells

试验采用外周血红细胞微核测试技术,通过分析鳝鱼外周血细胞的核异常率和微核率,研究多氯代甲烷的遗传毒性和生态安全性。结果表明:①96h后,质量浓度为3mg·L-1,30mg·L-1和300mg·L-1的CCl4和CHCl3污染所产生的核异常率分别为:5.38%和4.26%,9.62%和6.50%,12.18%和9.54%,表明CCl4对细胞异常率的影响大于CHCl3对细胞核异常率的影响;②96h后,质量浓度为3mg·L-1,30mg·L-1和300mg·L-1的CCl4和CHCl3污染所产生的微核率分别为:0.64%和0.40%,0.94%和0.55%,1.31%和0.95%,说明CCl4对细胞微核率的影响大于CHCl3对细胞核微核率的影响。表明用微核测试的方法分析环境中的污染物对生物体危害的程度是一种最便捷和有效的手段。

By analyzing the nucleus abnormity rate and the micronucleus rate of the mud eel peripheral red blood cell with micronucleus monitoring technique, the experiment studied the genotoxicity and ecological safety of polychlorinated methane. Results indicated： first, after 96 hours, the nucleus abnormity rate was 5.38% and 4.26% ,9.62% and 6.50%, 12.18% and 9.54% respectively when the concentrations of CCl4 and CHCl3 were 3 mg. L^-1, 30 mg.L^-1 和 300 mg. L^-1, indicating the effect of CCl4 on the nucleus abnormity rate is more obvious than that of CHCl3; second, after 96 hours, the micronucleus rate was 0.64% and 0.40%,0.94% and 0.55%, 1.31% and 0.95% respectively when the concentrations of CCl4 and CHCl3 were 3 mg.L^-1,30 mg.L^-1 和 300 mg.L^-1, indicating the effect of CCl4 on the micronucleus rate is more obvious than that of CHCl3. Harm degree of pollutants in the environment analyzed by micronucleus monitoring method is suggested to be one of the most facile and effective methods.