EPA和DHA对断奶仔猪淋巴细胞转化和细胞内信号分子的影响

Effect of EPA and DHA on Lymphocyte Proliferation and Intracellular Signaling Molecules in Weanling Pigs

研究二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对断奶仔猪外周血淋巴细胞转化及相关细胞内信号分子的影响。结果表明:(1)EPA和DHA剂量依赖性地抑制淋巴细胞转化(P<0.05);(2)EPA和DHA剂量依赖性地刺激一氧化氮(P<0.001)、环腺苷酸(P<0.001)和环鸟苷酸(P<0.001)的产生,提高环腺苷酸/环鸟苷酸比例(P<0.05);(3)EPA和DHA使细胞内游离Ca2+浓度出现瞬间上升,其上升幅度有明显的剂量依赖关系;(4)EPA和DHA剂量依赖性地抑制蛋白激酶C活性(P<0.05),而对蛋白激酶A活性无影响。EPA和DHA抑制仔猪淋巴细胞功能,其作用可能是通过改变细胞内信号转导实现的。

The experiment was conducted to investigate the effect of eicosapentaenoic acid （EPA） and docosahexaenoic acid （DHA） on peripheral blood lymphocyte proliferation and intracellular signaling molecules in weanling pigs. The results showed that EPA and DHA inhibited lymphocyte proliferation （P 〈 0.05） and stimulated nitric oxide （NO） （P 〈 0. 001）, cAMP （P 〈0. 001） and cGMP （P 〈 0. 001） production and cAMP/cGMP ratio in a dose-dependent manner. EPA and DHA induced a momentary rise of intracellular Ca^2＋ , and the rising extent was dependent on the concentration of EPA and DHA. EPA and DHA also inhibited protein kinase C activity in a dose-dependent manner （P〈 0.05）, and had no effect on protein kinase A activity. These results suggested that EPA and DHA exert an inhibitory effect on lymphocyte function, possibly due to alteration of intracellular signal transduction.