二重PCR检测猪、鸡源致病性大肠杆菌、沙门氏菌磺胺类耐药基因(Sul1、Sul2、Sul3)的研究

Detection of Sulfonamides-Resistant Genes of Pathogenic Salmonella and E.coli by Duplex PCR

利用二重PCR对临床分离的猪、鸡源致病性大肠杆菌103株和沙门氏菌33株的3个磺胺类药主要耐药基因Sul1、Sul2、Sul3进行了检测。结果表明,在19株耐SD的沙门氏菌中有17株检测出Sul1或Sul2基因,检出率为89.47%,与药敏试验的结果相比,符合率为89.47%;在56株耐SD的大肠杆菌中有53株检出耐药基因,与药敏试验的符合率为94.64%。二重PCR方法与常规PCR的结果符合率为98.5%,具有很高的一致性;与药敏试验也有很好的符合率,具有较高的特异性。整个试验过程只需7 h,比常规PCR节约7 h,检测速度有了较大的提高。

The Sulfonamides-resistant genes Sul1 ,Sul2, Sul3 were detected by duplex PCR from 33 Salmonella strains and 103 E. coli strains from pig and chicken. The results showed that Sul1,Sul2 genes of 17 Salmonella strains were detected from 19 SD resistant Salmonella strains demonstrated by MIC, and the detection rate was 89.47%. The coincidence rate between duplex PCR assays and MIC assays was 89.47%. Sul1 ,Sul2 genes of 53 E. coli strains were detected from 56 SD resistant E. coli strains. The rate of coincidence of duplex PCR assays and MIC assays was 94.64%. The rate of coincidence between duplex PCR assays and common PCR assays is 98.5%. It was only taken 7 hours to complete the whole duplex PCR test.