摘要
用嗜水气单胞菌产生的细胞毒素—溶血素Ah-1,裂解羊泰勒虫感染的红细胞,通过Percoll密度梯度离心分离羊泰勒虫裂殖子,建立了一种自感染红细胞中分离泰勒虫裂殖子的快速方法。光学显微镜观察显示,所分离的羊泰勒虫裂殖子基本保留原有的正常形态,并从宿主细胞成分中游离。
A simple and rapid method for purifying merozoites of Theileria spp. from infected erythrocytes in sheep or goats was developed. Infected erythrocytes were effectively lysed using hemolysin Ah-1 produced by Aeromonas hydrophila, and the lysate was subjected to centrifugation in a Percoll discontinuous density gradient at common speed. Light microscopical study showed that the merozoites purified retained their intact basic structure and were free of infected erythrocyte membrane and other host-cell components.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2007年第10期1093-1097,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家"863"计划(2006AA10A207)
国家自然科学面上项目(30571397)
欧盟EPIZONE
ICTTD和SSA-INCOME项目(INCONo.510561)
关键词
羊泰勒虫
裂殖子
溶血素
嗜水气单胞菌
Theileria spp.
merozoite
hemolysin Ah-1
Aeromonas hydrophila
