期刊文献+

用溶血素从感染红细胞分离羊泰勒虫裂殖子 被引量:2

Purification of Merozoites of Theileria spp. from Infected Erythrocytes in Sheep Using Hemolysin
下载PDF
导出
摘要 用嗜水气单胞菌产生的细胞毒素—溶血素Ah-1,裂解羊泰勒虫感染的红细胞,通过Percoll密度梯度离心分离羊泰勒虫裂殖子,建立了一种自感染红细胞中分离泰勒虫裂殖子的快速方法。光学显微镜观察显示,所分离的羊泰勒虫裂殖子基本保留原有的正常形态,并从宿主细胞成分中游离。 A simple and rapid method for purifying merozoites of Theileria spp. from infected erythrocytes in sheep or goats was developed. Infected erythrocytes were effectively lysed using hemolysin Ah-1 produced by Aeromonas hydrophila, and the lysate was subjected to centrifugation in a Percoll discontinuous density gradient at common speed. Light microscopical study showed that the merozoites purified retained their intact basic structure and were free of infected erythrocyte membrane and other host-cell components.
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2007年第10期1093-1097,共5页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金 国家"863"计划(2006AA10A207) 国家自然科学面上项目(30571397) 欧盟EPIZONE ICTTD和SSA-INCOME项目(INCONo.510561)
关键词 羊泰勒虫 裂殖子 溶血素 嗜水气单胞菌 Theileria spp. merozoite hemolysin Ah-1 Aeromonas hydrophila
  • 相关文献

参考文献17

  • 1Neitz W O. Theileriosis, gonderiosis and cytauzoonosis: a review[J]. Onderstepoort Journal of Veterinary Research, 1957, 27: 257-430.
  • 2Mehlhorn H, Schein E, Ahmed J S. Theileria[M]. London: Academic Press, 1994. 217-304.
  • 3Barnett S F. Theileria[M]. New York: Academic Press, 1977. 77-113.
  • 4吕文顺,魏宝瑛,黄守云,等.牛泰勒焦虫病的研究[M].银川:宁夏人民出版社,1980.26-33.
  • 5Friedhoff K T. Tick-borne diseases of sheep and goats caused by Babesia, Theileria or Anaplasma spp. [J]. Parassitologia, 1997, 39: 99-109.
  • 6Luo J X, Yin H. Theileriosis of sheep and goats in China[J]. Tropical Animal Health and Production, 1997, 29: 8s-10s.
  • 7王长吉.牛梨形虫病[M].北京:科学出版社,1993.381-390.
  • 8Sugimoto C, Sato M, Kawazu S, et al. Purification of merozoites of Theileria sergenti from infected bovineerythrocytes[J]. Parasitol Res, 1991, 77 (2): 129-131.
  • 9Asao T, Kinoshita Y, Kozaki S, et al. Purification and some properties of Aeromonas hydrophila hemolysin[J]. Infect Immun, 1984, 46(1): 122-127.
  • 10Hamburger J, Kreier J P. The isolation of Malaria parasites and their constituents[M]. New York: Academic Press, 1980.1-65.

同被引文献33

  • 1郭淑珍,马登录,牟永娟,杨树猛,王文飙,格桂花,尕旦吉,方百乾,赵春生.甘南地区羊泰勒虫病的预防与治疗试验[J].中国兽医寄生虫病,2006,14(3):48-51. 被引量:15
  • 2盛明宏,于建敏,王志亮,张西臣,吴鑑三.羊泰勒虫PCR检测方法的建立和初步应用[J].中国动物检疫,2007,24(7):20-22. 被引量:6
  • 3王奉先 李欧 等.湟源、门源、尖扎三县羊泰勒虫病调查[J].畜牧兽医科技通讯,1980,(3):29-32.
  • 4杨辅国 冯泽光 等.甘孜藏族自治州乾宁农牧站绵羊泰勒焦虫病报告[J].中国兽医杂志,1958,(2):33-37.
  • 5李作民 郝有奎 陈廷和 等.固原地区羊泰勒焦虫病的报告.中国兽医科技,1985,(11):81-85.
  • 6Leemans I, Hooshmand-Rad P, Ug-gla A. The indirect fluorescent antibody of the sheep parasite Theileria lesto-quardi [J]. Veterinary Parasitology, 1997, 69: 9-18.
  • 7青海畜牧兽医科学研究所.山羊泰勒焦虫病的诊断.青海畜牧兽医科学研究所资料汇编,1968,5:273-276.
  • 8Li Z M, Hao Y K, Chen T H, Yu X Q, Xi H L, Zuo Y R, Chen Y Q Liu J. Investigation on ovine theileriosis in Longde County, Ningxia province. Journal of Gansu Animal Science and Veterinary Medicine 1984, (1): 81-85. (in Chinese)
  • 9Luo J X, Yin H. Theileriosis of sheep and goats in China. Tropical Animal Health and Production, 1997, 29: 8-10.
  • 10Uilenburg CL International collaborative research: significance of tick-borne hemoparasitic diseases to world animal health. Veterinary Parasitology, 1995, 57: 19-41.

引证文献2

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部