摘要
为了研究共刺激分子CD80(B7-1)、CD86(B7-2)在流产发生机制中的意义,探讨中药黄芩及其成分的安胎作用及机理,本试验用米非司酮(RU486)皮下注射(每鼠90μg)诱导BALB/c小鼠流产,流式细胞仪测定脾脏中CD80+、CD86+细胞的数量。发现RU486诱导流产的小鼠脾脏中CD80+细胞显著升高,CD86+细胞显著减少。预先经口给予保胎中药黄芩及其单体成分黄芩苷和黄芩素,则能显著抑制RU486的流产作用,使母鼠胚胎吸收率降低,脾脏中CD80+细胞数量显著降低,CD86+细胞数量显著升高。这些结果表明,RU486诱导流产与机体共刺激分子CD80+、CD86+有关。保胎中药黄芩及单体成分有调节共刺激分子CD80+、CD86+细胞数量的作用。
In order to investigate the significance of the co-stimulatory molecules CD80 （B7-1）/ CD86（B7-2） in abortion and the anti-abortion mechanisms of Scutellaria baicalensis Georgi and its components, mifepristone （90μg per mouse） was injected subcutaneously （sc） to induce abortion in BALB/c mice. The number of CD80^＋ and CD86^＋ cells in the spleen were assayed by flow cytometry. The results revealed that the CD80^＋ cells significantly elevated, however, the CD86^＋ cells significantly decreased in group B （the group treated with RU486 ） in comparison with group A （the control group）. The decoction of Scutellaria baicalensis Georgi, and its components （baicalin or baicalein） given orally to the pregnant mice prior to RU486 treatment group significantly inhibited the abortifacient effect of RU486, and its CD80^＋ cells number significantly decreased, the CD86^＋ cells number significantly elevated in comparison with the group B. These results suggested that RU486 inducing abortion associated with the CD80^＋ and CD86^＋ cells number in the spleen, and that the anti-abortion effects of the Scutellaria baicalensis Georgii, its componetnts （baicalin or baicalein） are realized through modulation of the number of CD80^＋ and CD86^＋ cells in the spleen.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2007年第10期1131-1135,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(30571361)
