α1b-干扰素对白蛋白诱导大鼠免疫性肝纤维化的抑制作用

Inhibition of interferon-α1b on human albumin-induced hepatic fibrosis in rats

目的观察干扰素对白蛋白诱导的大鼠肝纤维化形成的影响。方法采用人血白蛋白诱导大鼠肝纤维化模型,并随机给予大剂量、小剂量干扰素、秋水仙碱处理。观察血清ALT和AST活性、总蛋白(TP),白蛋白(Alb)含量和球蛋白和白蛋白比值。放射免疫分析法测定层连蛋白(LN)、透明质酸(HA)、IV型胶原(Ⅳ-C)的含量,化学法测定肝脏羟脯氨酸(HyP)的含量。光镜观察肝纤维化程度。结果干扰素能明显降低人血白蛋白诱导的肝纤维化大鼠血清ALT、AST活性(P<0.05),提高Alb、TP含量和A/G比值(P<0.05),降低血清LN、HA和Ⅳ-C含量(P<0.05)。病理学观察发现,干扰素能使大鼠胶原纤维沉积明显减轻,假小叶结构明显减少,并明显降低纤维化计分(P<0.05)。结论干扰素可抑制人血白蛋白诱导的大鼠肝纤维化形成和发展。

Objective To observe the effects of interferon-α1b on human albumin-induced hepatic fibrosis in rats. Methods The human albumin-induced hepatic fibrosis was made in rats and the animals were treated with interferon-α1b and colchicines. The serum hepatic fibrotic markers and the liver histopathological examination were routinely conducted. Results Interferon-α1b decreased the serum ALT and AST levels, increased the serum albumin level, decreased the LN, HA, IV-C and HyP levels, and improved the liver fibrosis histologically. Conclusion Interferon-α1b can inhibit the hu- man albumin-induced hepatic fibrosis in rats.