气电纺制备新型纳米材料及骨细胞相容性研究

Preparation of Novel Nanometer Materials by Gas-jet/electrospinning and Study of Their Biocompatibility

探索纳米羟基磷灰石(n-HA)与聚醚砜(PES)电纺的最佳比例,制备新型纳米材料,并探讨其骨细胞相容性。分别以n-HA/PES:20/80,15/85,10/90(质量分数,下同),PES22%为电纺的比例,用N,N-二甲基甲酰胺(DMF)作溶剂,通过气电纺的方法制备了纳米羟基磷灰石与聚醚砜的复合纳米材料,运用扫描电镜、X射线能谱分析等方法进行检测,并在制得的纳米材料上接种成骨细胞,发现当n-HA与PES比例为10/90时,纺丝效果较好,纤维分布均匀:接种成骨细胞后,与阴性对照组比较,细胞在增殖上具有优势。从而证明气电纺制备的n-HA/PES纳米材料具有良好的骨细胞相容性。

A novel nanofibrous matrix from nanometer hydroxyapatite （n-HA） and polyethersulfone（PES） was fabricated, and its biocompatibility was examined by culturing osteoblast on the scaffolds. Nanometer hydroxyapatite with polyethersulfone ratio of 20 to 80, 15 to 85, 10 to 90 has been gas-jet/electrospun into nanofibers, respectively. The n-HA/PES scaffold was characterized by scanning electron microscopy （SEM） and X--ray energy dispersive microanalysis （EDX）. It was found that the nanofibers with a n-HA/PES ratio of 10/90 showed smaller diameter and narrower diameter distribution than the others. Experimental results from MTT assay and SEM imaging showed that the composite nanofibrous scaffold promoted the attachment and proliferation of the osteoblast. Thus, n-HA/PES composite nanometer materials may be a useful scaffolding material for bone tissue engineering.