气-电纺PHB引导组织再生膜的仿生矿化研究

Biomineralization of Air/Jet-Electrospun PHB GTR Membrane

实验将PHB通过气流—高压静电纺丝制成无纺膜,利用扫描电镜(SEM)、X射线衍射(XRD)对其形态和结构进行表征。在体外研究中,分别将无纺膜样本浸入37℃1.5倍仿生体液(SBF)中3、7、14、28 d后对其表面形态和结构进行表征。结果表明,气电纺PHB无纺膜由超细纤维交织而成,纤维之间有多孔形成:通过气流—静电纺丝之后,PHB的结晶度及晶体的有序度降低;PHB膜在SBF中浸泡3天之后就有矿化结晶在纤维的表面形成,随着浸泡时间的增长,矿化结晶的数目增加。结晶度也随之增高,XRD及FTIR表明形成的结晶为含有碳酸根的磷灰石晶体。

In order to explore a new useful porch to improve the performance of PHB-based GTR membrane, membrane was manufactured through the air/jet electrospinning process which can potentially generate nanometer scale diameter fibers and enlarge surface area of materials while maintaining high porosity. Successively, the biomineralization behavior of the membrane in simulated body fluid （SBF） was studied. The results of this investigation show that the successfully manufactured porous PHB membrane has high activity in SBF. It can be concluded that the novel technology could improve the performance of the PHB-based GTR membrane, and air/jet-electrospun PHB membrane has the potentiality to be used in guiding bone regeneration.