固定化锌螯合物亲合色谱填料的合成及其色谱行为

Syntheses and Characterization of Schiff Bases of 3,4 Dibutyltindioxybenzaldehyde with Aromatic Amines

以大孔微球硅胶为基质，用两种方法合成了高效锌离子螯合物亲合色谱填料。第一种是将硅胶经γ－缩甘油丙基三甲氧基硅烷活化后与亚氨基二乙酸键合；第二种是先将γ－缩甘油丙基三甲氧基硅烷与亚氨基二乙酸反应，再键合到硅胶上。然后与锌离子螯合，得到锌离子螯合物亲合色谱填料。对这两种填料的性质和色谱行为进行了研究。从对标准蛋白分离的结果来看。

Dibutyltindioxybenzaldehyde has been prepared by the reaction of 3,4 dihydroxybenz aldehyde with dibytyltin oxide and 4 Schiff bases synthesized by the condensation of the obtained aldehyde with aromatic primary amines. All products are characterized through elemental analysis, IR and 1H NMR spectra.