显微光照下的大鼠光化学血栓形成

Rat Photochemical Thrombogenesis under Microscopic Light

显微光照下的大鼠光化学血栓形成／彭健，廖福龙，李文／中国中医研究院中药研究所，北京１００７００介绍一种最近研制的简便的大鼠单支微静脉血栓形成模型。该方法由廖福龙设计，以显微镜光源兼作照明及光化学反应诱导光源，采用普通生物显微镜及摄录像系统，监测大鼠（...

A simplified micro photochemical induced thrombogenesis model is recently developed. Microscope with video camera and recorder were applied for monitoring mesentery microcirculation of Wistar rats (80￣100g) . According to the design of Prof. Liao FL, the microscopic light(halogen bulb, 100W) was localized by a specially made aperture to illuminate and to irradiate a selected single venule with the venule diameter about 40μm and blood velocity within 0.8￣1.1mm/s . In the meantime, the photochemical thrombogenesis was induced by irradiation at the level of about 10 8lx incorporated with rose bengal (125mg/kg) injection. Duration between dye injection and irradiation is recommended among 5￣25min . Platelet thrombogenesis appeared, gradually got thick, and eventually blocked the single venule, vessel blockage time(VBT) were dye dose, light intensity dependent. The results showed that VBT was controlled at 460±123s and Action know drug, ligustrazine, effectively prolonged VBT to 676±167s ( P <0.001) . It indicated that this simplified method can be used for pharmacological application.