超氧化物歧化酶和维生素E对新生大鼠缺氧缺血脑组织脂质过氧化物的影响

STUDY ON THE CHANGES OF GLUTAMATE RECEPTOR OF NEWBORN PIG'S BRAIN TISSUE IN HYPOXIC-ISCHEMIC CEREBRAL INJURY

本研究通过给新生缺氧缺血脑病（HIE）大鼠腹腔注射不同剂量的超氧化物歧化酶（SOD）、维生素E（VitE）加维生素C（VitC），观察这些药物对HIE大鼠脑组织脂质过氧化物（LPO）的影响：分别用于大剂量的SOD和VitE＋VitC治疗时，HIE大鼠脑内LPO水平与仅注射生理盐水组相比无明显下降甚至增加（SOD大剂量组）。当将SOD和VitE＋VitC剂量分别降低10倍和2倍时，HIE鼠脑LPO水平明显降低并接近正常组。表明适当剂量的SOD及VitE＋VitC可以通过降低脑内LPO水平而减轻由缺氧缺血所致的脑损伤。但当其剂量过大时反而有可能加重这一损伤。

The newborn pig's forebrain crude synaptic membrane (SPM) was used as receptor reagent to test the changes of glutamate receptor (Glu R) in the following three groups: control group (group A), Hypoxic-ischemic encephalopathy (HIE) 2 hours group (group B)and HIE 24 hours group (group C). The results showed that the mean receptor density (Bmax) in group C was significantly lower than that in group A and group B. which was of significant statistic difference (P<0.01 ). There was no difference between group A and group B. The affinity (Kd) of Glu R in these three groups showed no any statictic difference. The resuts of this research suggested that Glu R do involve in the pathogenesis of Hypoxic-Ischemic cerbral injury in newborn animals