中华眼镜蛇毒神经生长因子的研究

The Study of Nerve Growth Factor in Chinese Cobra

采用ＣＭ－ＳｅｐｈａｒｏｓｅＣＬ－６Ｂ和ＳｅｐｈｏｓｉｌＣ８以及ＨＰＬＣ层析方法，自广西产中华眼镜蛇毒中分离纯化神经生长因子（ＮＧＦ），此ＮＧＦ经８ｄ鸡胚背根神经节体外培养证明具有促进神经纤维生长的活性。经ＨＰＬＣ及聚丙烯酰胺凝胶电泳鉴定为单一组成，经ＳＤＳＰＡＧＥ及ＨＰＬＣ测定分子量分别为２４．１ＫＤ和２４．９ＫＤ，等电聚焦电泳测得ｐＩ为８．２，氨基酸组分分析表明ＮＧＦ含酸性氨基酸较少，通过１２５｜标记ＮＧＦ测定出ＮＧＦ在生物体内主要分布于肾、肺。

Nerve growth factor (NGF)was purified from the venom of Chinese Cobra (Naja naja atra) by the CM Sepharose CL 6B column chromatography and sephasil C8 reversed phase chromatography,and then by the HPLC. The purified NGF was shown to be homogeneous in discontinuous PAGE,in SDS PAGE and in High pressure Liquid Chromatography (HPLC). In vitro, the greatest outgrowth of the dorsal root ganglias of chicken embryos invoked by NGF at a concentration of 100 ng/mL. The molecular weight was estimated to be about 24 9 KD by HPLC, and to be 24 1 KD by SDS PAGE. The isoelectric point was about 8 2. Amino acid analysis showed that NGF contained less residues of acidic amino acid.In vivo, the distribution of 125 | NGF was found to be concentrated in kidney, peripheral nerve, liver and lung.