大鼠视觉可塑性关键期终止相关基因的筛选

The genes associated with termination of the critical period of nuroplasticity in visual cortex

目的筛选视觉可塑性关键期终止相关基因，探讨视觉可塑性关键期终止的分子机制。方法采用暗饲养及暗饲养后加光照刺激动物模型，应用抑制性消减杂交技术构建大鼠视皮层与视觉可塑性关键期终止相关的cDNA文库。并通过PCR筛选、反向Northem杂交验证、测序及同源性检索对差异表达基因进行分析。结果成功构建了高消减效率的大鼠视皮层与视觉可塑性关键期终止相关的cDNA文库，经筛选、测序及同源性分析，得到14个视觉可塑性关键期终止时视皮层上调表达基因的片段。其中13个为已知基因，除B—Tubulin、Mbp、Cyclophilin基因有文献报道跟可塑性有关外，其余基因与视觉可塑性的关系以往少有报道。结论通过大鼠视皮层与视觉可塑性关键期终止相关的cDNA文库的建立，筛选出一批与视觉可塑性关键期终止相关的候选基因，为进一步阐明视觉可塑性关键期终止的分子机制提供了重要的基础。

Objective To screening genes associated with termination of the critical period of neuroplasticity in visual cortex and investigate the molecular mechanism. Methods To construct a eDNA library using visual cortex of dark rearing rats and normal light rearing 1 week after dark rearing 60 days rats with suppression subtractive hybridization technique. PCR, reverse No,them hybridization, sequencing and homology search were used to analysis the differential expression genes fragments. Results The eDNA library of termination the critical period was set up successfully. After screening, sequencing and homology search 14 sequences were obtained. Among of the genes 13 were known genes and one was novel gene fragment. They were up-regulated genes in visual cortex when the critical period termination. Conclusions Through construction the eDNA library, we screened a crop of candidate genes related to termination the critical period. The results provided important foundation to further illuminate the molecular mechanism of termination the critical period of neuroplasticity in visual cortex.