麝香乌龙丸对卵巢切除大鼠股骨骨折愈合的实验研究

The study of mask dark dragon pelle on femural fracture healing in ovariectomized rats

目的研究麝香乌龙丸对卵巢切除大鼠股骨干骨折愈合的作用。方法将30只雌性、12周龄Sprague-Dawley大鼠分成3组假手术组(Sham+F+V)、卵巢切除+骨折+生理盐水对照组(OVX+F+V)、卵巢切除+骨折+麝香乌龙丸给药组(OVX+F+M),每组10只大鼠。所有需制造骨折的大鼠均采用右股骨中段横行骨折,髓内针固定;麝香乌龙丸给药组采用大鼠灌胃给药(1.2g.kg-1.d-1),于术后4周杀死,取大鼠右侧股骨标本;分别进行CR摄片、组织形态学染色,并应用双能X线骨密度仪(DEXA)测量右股骨整体骨密度(tBMD)、远段骨密度(dBMD)和中段骨密度(mBMD)以及BMP-2免疫组化观察,并应用病理图像分析仪对BMP-2免疫组化进行光密度测定。结果麝香乌龙丸给药组与OVX+F+V比较,前者骨痂mBMD和BMP-2的表达显著增高,骨小梁增宽、排列较整齐,板层骨形成,软骨组织可见。结论麝香乌龙丸对OVX大鼠股骨骨折有明显促进骨折愈合的作用,并加快编织骨向板层骨的演变过程,这与BMP-2的表达有明显相关性。

Objective To evaluate the effects of Mask Dark Dragon Pelle on femur fracture healing in bilateral ovariectomized rats. Methods Thirty 12-week-old female Sprague-Dawley rats were divided randomly into three groups of ten animals each. Group A was sham-operated and femur fracture and vehicle group（Sham ＋ F ＋ V）, Group B was bilateral ovariectomized and femur fracture and vehicle group （OVX ＋ F ＋ V ）and Group C was bilateral ovariectomized and femur fracture and administration of Mask Dark Dragon Pelle group（ OVX ＋ F ＋ M）. Mask Dark Dragon Pelle was administered once everyday （ 1.2 g/kg） for 4 weeks. Right femoral diaphysis was fractured transversely at the midshaft and fixed with intramedullary stainless wire. All rats were sacrificed 4 weeks after operation. Right femora were removed for the observation of radiology and histology, and for the monitoring of bone mineral density （BMD） by dual-energy X-ray absorptiometry （DEXA）, immunohistochemistry assessment and Integral Optic Density（IOD）. Results The mean mBMD and IOD of the OVX ＋ F ＋ M group was significantly higher than that of the OVX ＋ F ＋ V group. The woven bones have remodeled into lamellar bones and cartilage islands were observed. Conclusions Mask dark dragon pelle accelerates the fracture healing in ovariectomied rats and improves woven bones to remodel into lamellar bones. The fracture healing is in accordance with the expression of BMP-2.