6-BA和IBA对丹波黑豆愈伤组织黄酮产量影响研究

Study on Effect of 6-BA and IBA on Flavonoid Production of Danbo Black Soybean Callus

以丹波黑豆下胚轴为外植体,接种到1/2MSN6(MS无机盐+N6有机盐)上培养出一致的愈伤组织,将一定量的愈伤组织接种到不同浓度的6-BA(6-苄氨基腺嘌呤)和IBA(吲哆-3-丁酸)组合的培养基上培养15天后,称重,计算愈伤组织生物产量,并取出烘干至恒重,称量其干重。提取愈伤组织中黄酮,分别测定芦丁和槲皮素的生物产量。结果表明:(1)2mg/L的6-BA处理,愈伤组织产量最高,高于其他浓度下的产量,6-BA与IBA相互作用对愈伤组织生物产量有显著影响;(2)2mg/L的6-BA处理,芦丁生物产量和含量最高,并显著高于其他浓度下的芦丁生物产量;(3)2mg/L的6-BA处理,槲皮素生物产量和含量最高,并且其槲皮素生物产量随培养基中IBA浓度的增加而降低;(4)愈伤组织生物产量与芦丁黄酮和槲皮素黄酮生物产量都存在显著的正相关;(5)愈伤组织中槲皮素生物产量和芦丁生物产量之间也存在着显著的正相关。

Below hypocotyls of danbo black soybean were selected as explant and inoculated in 1/2 MSN6 （ inorganic of MS ＋ organic of N6 ） medium and acquired identical callus. Quantitative identical callus were inoculated in medium with different concentration of 6-BA and IBA for 15 days, callus weighed, and dried and weighed up. Flavonoid were extracted from callus and measured biological yield of rutin and quercetin. The results showde that biological yield of callus were the weightest in culture medium of 2 mg/L 6-BA ; and bilolgical yield of callus were influencde by interaction of different concentration of 6-BA and IBA ; biological yield of tu- rin were the higest in culture medium of 2 mg/L 6-BA ; biological yield of quercetin were the higest in culture medium of 2 mg/L 6-BA, and biological yield of quercetin became less and less while contention of IBA increasing;biological yield of turin and quercetin were remarkably positive related to biological yield of callus; biological yield of quercetin was significantly positive related to biological yield of turin.