LFS和CPPU在罗汉果组培中生物效应的对比研究

Contrast Study of Bioeffects Between LFS and CPPU on the Tissue Culture of Siraitia Grosvenori(Swingle)C.Jeffrey in Vitro

为给罗汉果组培技术的改进提供实验证据,以罗汉果单芽茎段作外植体,以MS为基本培养基,分别添加LFS和CPPU等生长调节剂。结果:(1)在原始外植体培养中,LFS单独使用能诱导腋芽迅速萌发生长,多数可发生不定根,愈伤组织则很少。CPPU单独使用,只能诱导愈伤组织迅速发生与大量增殖,不能使腋芽萌发成苗。(2)在继代增殖培养中,LFS促进腋芽萌发生长和不定根发生的活性均能被NAA增强。CPPU复配NAA后,亦可诱导腋芽萌发,并促进叶片迅速长大,然而在形成大量愈伤组织的同时,严重抑制茎的伸长与不定根的发生。

In order to improve the tissue culture technology of Siraitia grosvenorii （Swingle）, stem cutting with single nod was used as explants, MS as basal medium and supplied with LFS or CPPU respectively. Resuits ： （ 1 ） On the primary culture LFS 0.2 （ mg/L, as same as fallows ） could induce the axillary bud of explants germinating and growing quickly, most of them could rooting, meanwhile the callus formation was controlled obviously during 25 d cultured. But CPPU 0.02 could only induce a lout of callus formation, no bud germinated. （2） On the secondary culture for proliferation the bioactive of LFS to induce axillary bud germinating and plantlet rooting was enhanced by cooperation with NAA 0.1. At the same time on the help of NAA, CPPU also induced the axillary bud germinating and improved leaves growing bigger, however stem elongation and adventitious roots are strongly inhibited at the same time large numbers of callus are formde.