尖锐湿疣皮损朗格汉斯细胞的形态学变化

Morphological Features of Langerhans' Cells in Lesions of Condyloma Acuminatum

目的了解尖锐湿疣(CA)皮损朗格汉斯细胞(LCs)的形态学的变化。方法采用免疫组化法对13例CA患者的CA皮损及其正常皮肤的LCs进行CD1a染色,光镜下对CD1a+LCs进行计数并观察其形态和分布;使用电镜对其中6例患者的CA皮损及其正常皮肤的LCs进行超微结构观察。结果与正常皮肤相比,CD1a+LCs大多散在分布于表皮下部,突起明显减少、缩短或消失;半定量计数显示CA表皮LCs数目(26.31±18.84)较正常皮肤LCs数目(72.00±27.40)明显减少(P<0.001)。电镜观察发现,CA表皮LCs的朗格汉斯颗粒数目(4.00±2.94)较正常皮肤朗格汉斯颗粒的数目(10.80±4.78)明显减少(P<0.001),并且大多LCs的超微结构形态异常,呈现变性改变。结论CA皮损LCs数目明显减少,且大多LCs呈现变性的形态学变化,这些变化可能提示CA皮损LCs的功能受损。

Objective To investigate the morphological features of Langerhans＇ cells （LCs） in condyloma acuminatum CA） lesions. Methods CD1a^＋LCs in 13 CA lesions and in 13 normal controls were labeled using immunohistochemistry and examined under light microscopy. Uhrastructural investigation on LCs in 6 CA lesions and in 6 normal controls was performed by electron microscopy. Results Compared with those in normal controls,most CD1a^＋LCs in CA lesions exhibited dysplastic dendrities and abnormal distribution. The number of CD1a^＋LCs in CA lesions （26.31 ± 18.84 ） was statistically lower （P 〈 0. 001 ） than that in normal controls （ 72.00 ± 27.40）. Electron microscopy revealed that the number of Birbeck granules within lesional LCs （4.00 ± 2.94 ） was significantly decreased （P 〈 0.001 ） than that in normal LCs （10.80 ± 4.78 ）. The uhrastructures of most lesional LCs displayed degenerative changes. Conclusion In CA lesions, the number of CD1a^＋ LCs was significantly decreased and the morphology of most LCs show degenerative changes, which suggest that these LCs have been functionally impaired.