应用基因芯片技术筛选肝细胞癌相关基因

Genechip used for screening differentially expressed genes in hepatocellular carcinoma

目的:探讨基因表达谱芯片技术在筛查肝细胞癌(HCC)相关基因群表达中的作用。方法:TRIzol法抽提2例HCC及正常肝脏组织总RNA,分离纯化两种组织的mRNA;逆转录合成掺入生物素标记的cDNA合成探针,与基因芯片(涵盖了18400个转录本,代表了14 500个明晰的基因)杂交,扫描芯片荧光信号图像,计算机分析,比较2种组织基因表达谱差异。结果:2例HCC组织与正常肝脏组织相比,有2756条基因(19.01%)共同表达差异,其中共同上调基因1772条和共同下调基因984条,对2756条共同差异表达基因作了初步功能分类,这些基因与HCC的发病机制存在相关性。结论:基因表达谱芯片技术可以筛选出HCC表达异常的相关基因群,有助于认识肝癌的发病机制。

AIM： To ascertain the effectiveness of genechip for screening the same differentially expressed genes of hepatocellular carcinoma （HCC）. METHODS： Total RNAs were isolated from tissues of two cases of HCC and one normal liver by the TRIzol method, and then purified, reverse-transcribed to cDNA incorporating a biotin-labeling probe, and hybridized with genechip （covering 18 400 transcripts, representing 14 500 distinct genes）. Picture signals of the fluorescence in the gene array were scanned and compared by computer analysis to the differential expression of the genes in the two tissues. RESULTS： The same differentially expressed genes were 2756 （19.01%） and the same up regulated and downregulated genes were 1772 and 984, respectively, in two HCC and one normal liver tissues. These genes were initially classifled according to function, and further analysis showed that they play an important role in the pathogenesis of HCC. CONCLUSION： The analysis of the gene expression profile of HCC using genechip can rapidly select associated abnormal genes, while further analysis of the obtained genes can help elucidate the pathogenicity of HCC.