摘要
目的:探讨檞皮素对大鼠酒精性脂肪肝的防治作用及其可能机制。方法:wistaur(?)鼠48只,随机分为:正常对照、酒精性脂肪肝模型、檞皮素处理、纳洛酮处理4组,实验4 wk末,处死所有大鼠,检测血AST,ALT和TNF-α,并行肝脏病理组织学及核转录因子(NF-ΚB)表达的检测。结果:檞皮素和纳洛酮组血AST,ALT和TNF-α明显低于模型组(檞皮素组:150.7±23.6 U/L,57.4±8.4 U/L,0.83±0.27μg/L;纳洛酮组:148.3±21.4 U/L,55.2±7.3 U/L,0.85±0.34μg/L vs 205.0±31.5 U/L,70.5±9.2 U/L,4.08±1.23μg/L;P<0.05),而高于正常对照组(127.6±17.8 U/L,47.9±7.1 U/L,0.22±0.10μg/L;P<0.05).檞皮素和纳洛酮组肝脏脂肪变程度较模型组轻,且懈皮素组肝内NF-ΚB表达水平显著低于模型组(60.27个/HP vs 28.49个/HP,P<0.05)。结论:檞皮素可能通过抑制NF-ΚB及TNF-α的表达,发挥防治酒精性脂肪肝的作用.
AIM： To explore the effect and possible mechanism of quercetin on alcoholic fatty liver （AFL） in rats.
METHODS： Forty-eight male wistar rats were randomly divided into four groups： normal control, model quercetin and naloxone groups. By the end of the fourth week, all rats were weighed, narcotized, sacrificed, and tested for expression levels of aspartate aminotransferase （AST）, alanine aminotransferase （ALT） and tumor necrosis factor-alpha （TNF-α） in plasma. Liver samples were analyzed for common histopathological changes and the expression of nuclear factor kappaB （NF-κB） was measured by immunohistochemical staining.
RESULTS： Levels of AST, ALT, and TNF-α in plasma were markedly lower in the quercetin and naloxone groups than the model group （quercetin, 150.7 ±23.6 U/L, 57.4± 8.4 U/L, 0.83 ± 0.27 μg/L; naloxone, 148.3 ± 21.4 U/L, 55.2 ± 7.3 U/L, 0.85± 0.34 μg/L vs 205.0 ± 31.5 U/L, 70.5 ± 9.2 U/L, 4.08± 1.23μg/L; P 〈 0.05）, but were higher in the quercetin and naloxone groups than in the normal contrast group （127.6 ± 17.8 U/L, 47.9± 7.1 U/L, 0.22± 0.10 μg/L; P 〈 0.05）. Compared with the model group, the degree of liver fatty degeneration in the quercetin and naloxone groups was slight, and the expression level of NF-κB in liver tissue was clearly lower in the quercetin group （60.27/HP vs 28.49/HP, P 〈 0.05）.
CONCLUSION： Quercetin may have a role in preventing alcoholic fatty liver by inhibiting expression of NF-κB and TNF-α.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第22期2399-2402,共4页
World Chinese Journal of Digestology
