血清甲状腺球蛋白及其自身抗体在化学发光免疫分析时的相互影响

Mutual interference between serum thyroglobulin and anti-thyroglbulin antibody in chemiluminescent immunoassay

目的探讨血清甲状腺球蛋白（Tg）及其自身抗体（TgAb）在化学发光免疫分析时的相互影响。方法在高浓度Tg血清中加入不同浓度TgAb血清,测定Tg回收率;在高浓度TgAb血清中加入不同浓度Tg血清,测定TgAb回收率。结果加入低、高浓度TgAb血清后,Tg回收率分别为93%-112%和17%-40%,Tg回收率和TgAb浓度呈负相关（rs=-0.877,P=0.001）;加入低、高浓度Tg血清后,TgAb回收率分别为76%-108%和66%-98%,TgAb回收率和Tg浓度呈负相关（rs=-0.881,P=0.004）。结论血清Tg和TgAb在化学发光免疫分析时存在相互影响,导致结果偏低并呈浓度依赖性,其中TgAb在低浓度时即对Tg产生显著干扰,而Tg在较高浓度时才对TgAb测定产生明显影响。

Objective To investigate the mutual interference between serum thyroglobulin（Tg） and anti-thyroglbulinantibody（TgAb） in chemiluminescent immunoassay（CLIA）. Methods Serum Tg and TgAb were measured by automated CLIA.Tg or TgAb recoveries were determined in serum samples blended with sera of different concentrations of TgAb or Tg. Results Tg recovery rate was 93%-112% in low TgAb sera,17%-40% in high TgAb sera with negative correlation with TgAb concentrations （rs=-0.877,P=0.001）,while TgAb recovery rate was 76%-108% in low Tg sera,66%-98% in high Tg sera with negative correlation with Tg concentrations （rs=-0.881,P=0.004）. Conclusions Mutual but not equal interferences between serum Tg and TgAb are present in CLIA,resulting in falsely low concentration in a concentration-dependent manner;TgAb interference existes even at low level and Tg interference existes only at high level.