肝移植巨细胞病毒感染发病受者CD8^+ IFN-γ^+和CD8^+ IL-4^+细胞比例的变化

Change of proportion of CD8^+ IFN-γ^+ cell and CD8^+ IL-4^+ cell in the patient with human cytomegalovirus infection after liver transplantation

目的分析肝移植术后人类巨细胞病毒(HCMV)感染发病受者外周血CD8+细胞内干扰素γ(IFN-γ)和白细胞介素4(IL-4)的变化。方法采用双色流式细胞术分析HCMV感染发病受者与HCMV感染未发病受者CD8+细胞中IFN-γ+和IL-4+细胞的水平。结果肝移植术后HCMV感染发病受者CD8+细胞中IFN-γ+细胞所占的比例较HCMV感染未发病受者低(P<0.05);CD8+细胞中IL-4+细胞在HCMV感染发病受者与HCMV感染未发病受者之间没有显著变化;IFN-γ/IL-4比值在HCMV感染发病受者明显降低。结论IFN-γ水平的降低与肝移植术后HCMV感染发病相关。

Objective To analyze the change of interferon-γ（IFN-γ） and interleukin-4（IL-4） in CD8^＋ cells in the patient with human cytomegalovirus（HCMV） infection after liver transplantation. Methods IFN-γ and IL-4 production by CD8^＋ cells in peripheral blood monouuclear cells（PBMC） from liver transplant recipients with and without HCMV infection were analyzed by immunofluorescence staining and two-color flow cytometry（FCM）. Results The IFN-γ of CD8^＋ cells in the patients with HCMV infection was significantly lower than that in patients without HCMV infection after liver transplantation（P〈0.05）.The IL-4 of CD8^＋ cells was similar in the patients with HCMV infection and that without HCMV infection.The ratio of IFN-γ/IL-4 was significantly lower in the patients with HCMV infection. Conclusions Low level of IFN-γ seems to correlate well with HCMV infection in liver transplantation recipient.