一种新型蚯蚓脱氧核糖核酸酶相对分子质量的测定

Molecular weight determination of a novel earthworm deoxyribonuclease

目的测定从蚯蚓组织中提取纯化的一种新型脱氧核糖核酸酶的相对分子质量。方法采用凝胶过滤层析法、十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法和基质辅助激光解吸附电离飞行时间质谱法三种方法测定一种新型蚯蚓脱氧核糖核酸酶(EWD1)的相对分子质量。结果凝胶过滤层析法、SDS-PAGE法和质谱法测定其相对分子质量分别为126440、136320和157190。结论EWD1由两个亚基组成,其相对分子质量最终确定为157190。

Objective To determine the molecular weight ot a novel deoxynbonuclease （EWD1） trom the earth-worm. Methods Gel filtration chromatography （GFC）, SDS-PAGE techniques and MALDI-TOF were employed, respectively, for this purpose. Results The molecular weights obtained with different measurements are 126440 （GFC）, 136320（SDS-PAGE） and 157190（MALDI-TOF）, respectively. Conclusion EWD; comprises two subunits, and its total molecular weight is confirmed to be 157190.