摘要
采用ELISA方法检测了实验室汰选的对Cry1Ab产生107倍抗性的亚洲玉米螟Ostrinia furnacalis(Guenée)种群与敏感种群3龄幼虫取食表达Cry1Ab杀虫蛋白的Bt玉米心叶后,杀虫蛋白在幼虫体内的分布情况。结果表明:Cry1Ab杀虫蛋白在抗性种群幼虫中的组织分布情况与敏感种群相近,主要存在于中肠组织和血淋巴中。抗、感种群中均以含有内含物的中肠组织中含量最高,分别为277.2 ng/g和104.9 ng/g;其次为血淋巴,分别为93.7 ng/g和69.5 ng/g;不含内含物的中肠组织中52.7 ng/g和40.1 ng/g;在丝腺和马氏管组织的含量很低,丝腺中分别为8.5ng/g和11.7ng/g,而马氏管中分别为6.7 ng/g和6.5 ng/g。脂肪体、生殖器官中未检测到杀虫蛋白。抗性种群中肠组织(含有内含物和不含内含物)中Cry1Ab的含量显著高于敏感种群。幼虫期取食过Bt玉米的亚洲玉米螟发育的蛹、成虫及其卵中均不含杀虫蛋白,说明Bt杀虫蛋白不会通过幼虫取食向蛹、成虫及卵传递。
The distribution and content of CrylAb insecticidal protein in different tissues of the Asian corn borer, Ostrinia furnacalis （Guenee）, when the 3rd instar larvae of CrylAb protein resistant and susceptible colonies fed on whorl leaves of Bt-transgenic corn expressing CrylAb protein for 3 days were determined and analyzed by enzyme-linked immunosorbent assay （ELISA）. The distributions of CrylAb in different tissues in the resistant and susceptible larvae were similar, mainly in the midgut tissue and haemolymph. The CrylAb contents in the midgut tissues of the resistant larvae with and without contents inside their midguts were 277.2 ng/g and 52.7 ng/g, respectively, significantly higher than those of the susceptible larvae, which were 104.9 ng/g and 40.1 ng/g, respectively. The CrylAb contents in the haemolymph of the resistant and susceptible larvae were 93.7 ng/g and 69.5 ng/g, respectively. The CrylAb protein in the silkgland and Malpighian tubules in the resistant and susceptible larvae could be detected, but the concentration was quite low. No CrylAb protein was detected in the fat body and reproductive organs, pupae, adults and eggs by ELISA method both in the resistant and susceptible larvae fed on the Bt corn. The results suggested that the CrylAb protein from larvae feeding on Bt corn could not be biotransferred to the subsequent pupae, adults and eggs.
出处
《昆虫学报》
CAS
CSCD
北大核心
2007年第9期957-961,共5页
Acta Entomologica Sinica
基金
国家自然科学基金项目(30370967
30471149)
