CXCR4受体在口腔鳞癌细胞系中的表达及对细胞增殖和迁徙的影响

Expression of CXCR4 and its modulation on migration,proliferation of oral squamous cell carcinoma cell line

目的:观察口腔鳞癌细胞系(OSCC)中趋化因子受体CXCR4的表达,检测SDF-1/CXCR4反应轴对OSCC增殖的作用,SDF-1对CXCR4阳性肿瘤细胞的趋化作用,探讨CXCR4受体在OSCC中的功能及活性。方法:细胞涂片免疫荧光法检测CXCR4蛋白在OSCC细胞系的表达,流式细胞仪直接免疫荧光法检测OSCC细胞系中CXCR4蛋白的表达量,MTT法检测细胞的增殖能力,体外迁徙实验检测SDF-1/CXCR4反应轴对OSCC细胞的趋化作用。采用SPSS10.0软件包进行ANOVA方差分析和t检验。结果:CXCR4蛋白在OSCC细胞系呈阳性表达,表达率为68.62%。OSCC细胞在SDF-l作用下,其增殖反应显著增强,CXCR4抗体可显著抑制肿瘤细胞的增殖,SDF-1可显著诱导OSCC细胞的移动。结论:CXCR4受体与OSCC细胞增殖、迁徙功能有一定关系。

PURPOSE： This study was aimed to evaluate the expression of chemokine receptors CXCR4 in oral squamous cell carcinoma cell line and to determine the role of CXCR4 in tumor cell proliferation and migration. METHODS： The expression of CXCR4 in oral squamous cell carcinoma cell line was evaluated by immunofluorescent staining and flow cytometry.MTT assay was used to determine the SDF-1/CXCR4 role on proliferation of tumor cells. Cell migration assays were carried out with the oral squamous cell carcinoma cell line to confirm the effect of SDF-1/CXCR4 on migration. SPSS 10.0 software package was used for ANOVA and Student＇s t test. RESULTS： Oral squamous cell carcinoma cell line displayed the CXCR4 expression with great intensity and the expression rate was 68.62%. In MTT assay, recombinant SDF-1 stimulated proliferation of OSCC cell and CXCR4 neutralization by monoclonal antibodies decreased proliferation. In migration assay, SDF-1 induced migration of OSCC cells. The migration cell mean of SDF-1 stimulated group was significantly higher than the control group （P〈0.05）. CONCLUSION： The expression of CXCR4 was significantly correlated with oral squamous cell carcinoma cell proliferation and migration.