HLA-DR位点基因分型在肾移植配型中的应用研究

Gene Typing HLA DR for Research of Renal Transplantation

应用聚合酶链式反应序列特异性引物（ＰＣＲＳＳＰ）技术，对３３１例肾移植供受者进行ＨＬＡＤＲ位点基因分型，其中１０９例同时与血清学分型进行对比研究。显示血清学方法误差率为２９．７％；而ＰＣＲＳＳＰ法的ＤＲ位点分型，所有特异性均能显示，无假阴性及假阳性。随机抽取的２０例样本进行重复实验，重复性为１００％。分型结果经ＩＮＮＯＬＩＰＡＰＣＲ反向ＳＳＯ实验确证，符合率为１００％。此方法耗时４ｈ，较血清学方法操作简便、快速、结果准确可靠，适合于临床器官移植ＨＬＡ分型。

In order to select donor and recipient,accurate typing HLA especially typing HLA DR is one of the most important factor for a longer survival time of the graft.Using polymerase chain reaction with sequence specific primers(PCR SSP) technique the authors underwent HLA DR gene typing in 331 cases of donor and recipient. Among them,109 cases were subjected to a comparative study with serological typing. The results showed that the error rate of serological assay was 29.7%, while the HLA DR gene typing with PCR SSP method all showed their specificities without any false negative or false positive.Replication experiments in 20 cases of random sampling showed 100% coincidence. The typing results were confirmed by INNO LIPA PCR Reverse SSO experiments,and the coincidence was 100%. Only 4 hours will suffice for this assay. So, it is simple, rapid and reliable as compared with the serological method, and is suitable for HLA typing in clinical organ transplantation.