摘要
目的为检验前期制备的新型生物玻璃/纳米羟基磷灰石涂层的细胞相容性。方法在本实验中,采用L929成纤维细胞在涂层浸提液中的培养,检测了涂层的细胞毒性,采用人体骨髓基质干细胞在涂层上的培养,检测了涂层对人体骨髓基质干细胞增殖和代谢的影响。结果低于浓度的涂层浸提液(<10%)对L929细胞增殖具有促进作用,而高浓度的涂层浸提液(>50%)对L929细胞的增殖具有抑制作用,其中100%的涂层浸提液对L929细胞的增殖抑制比例为20.9%,在细胞毒性分级中处于合格范围内。结论在培养早期,人体骨髓基质干细胞在涂层表面的增殖要优于对照组,涂层显示出良好的细胞相容性。由于合格的细胞毒性和良好的细胞相容性,该涂层的钛合金具有作为骨植入物的应用潜力。
Objective Bioactive glass/nanometer hydroxyapatite (BG/nHA) gradient coating was prepared on titanium alloy by low temperature melting in our precious work, and the coating showed better mechanical properties than that of the hydroxyapatite coating on titanium alloy by plasma-spraying method. As a new material, its cytotoxicity and cytoc- ompatibility must be evaluated before it is used in vivo. Methods In this work, the cytotoxicity was evaluated by culturing the L929 fibroblasts in the extraction solution of the coating (ESC), and the cytocompatibility was evaluated by culturing the human bone marrow stromal stem cell (HBMSC) on the surface of the coating for different periods. Results The results showed that the coating is low cytotoxic. The L929 fibroblasts density is higher in ESC with concentration lower than 10% than that in the controlling solution. Even in the 100% ESC, the cell density is 79.1% of that in the control solution. The cytotoxicity of the coating is located at level 1 according to the evaluation standard of cytotoxicity, and it is an acceptable level, Couelusion The coating also shows good cytocompatibility. The HMBSCs show higher proliferation rate on the surface of the coating than that on high density polyethylene in the early stage of cultivation. With the advantage of good mechanical properties, eligible cytotoxicity and good cytocompatibility, the titanium alloy with (BG/nHA) coating has great potential to be used as bone substitution material.
出处
《生物骨科材料与临床研究》
CAS
2007年第5期6-9,15,共5页
Orthopaedic Biomechanics Materials and Clinical Study
关键词
生物玻璃/羟基磷灰石复合涂层
钛合金
细胞相容性
Bioactive glass/hydroxyapatite composite coating
Titanium alloy
Cytocompatibility