基因工程菌JM109(pGEX-AZR)-小麦体系对酸性红B的生物处理

Biotreatment of Acid Red B by GEMJM109(pGEX-AZR)-associated Wheat

采用基因工程菌E.coliJM109(pGEX-AZR)-小麦联合生物体系,以酸性红B染料为例,研究了在模拟水培条件下联合体系对偶氮染料的生物处理效果。结果显示,联合处理时染料的脱色率高于基因工程菌和小麦的单独处理;两种生物体在单独处理时染料的脱色率随彼此生物量的增加而增加,并呈线性相关,相关系数分别为RGEM=0.973 6,R小麦=0.980 1。考察了温度、pH和有机碳源3种环境因素对联合体系生物处理的影响。结果表明,环境条件能明显改变体系对染料的处理,这种改变主要是通过影响体系内的基因工程菌实现的。

Using acid red B as example,the biotreatment of azo dyes by the system of GEMJM109 （pGEX- AZR）-associated wheat was studied. The results show that acid red B is decolorized more completely by the associated system than by separate system. With the increase of biomass of the organisms,decolorization rate of acid red B is also enhanced, showing a good linear relationship with RGEMs = 0. 973 6 and Rwheat = 0. 9801. We also studied the effect of environmental factors such as temperature ,pH value and nutrient source on the biotreatment system of GEMJM109 （pGEX -AZR）- associated wheat. It is found that alteration of environmental factors can influence the decolorization rate of acid red B ,and it acts mainly on JM109（ pGEX -AZR） of the associated system.