应用量子点纳米探针研究丹酚酸B与肿瘤细胞间的直接作用

Study on Interaction between Salvianolic Acid B and Cancer Cell by Quantum Dots-SAB Nanoscale Fluorescent Probes

目的应用量子点纳米荧光探针示踪中药有效成分与细胞之间的直接作用,为该技术成为一种研究平台提供实验依据。方法丹酚酸B(salvianolic acid B,SAB)经1-乙基-3(3-二甲基胺基丙基)碳二亚胺盐酸盐[1-ethyl-3(3-dimethylamino propyl)-carbodiimide,EDC]活化,以巯基乙胺(mercaptoethylamine,ME)为连接剂,与水溶性量子点(quantum dots,QDs)进行共价连接,将获得的QDs-SAB与肺腺癌细胞(SPCA-1)和肝癌细胞(7721)进行培育,通过QDs荧光直接观察SAB与细胞的结合情况,进而根据细胞形态观察、MTT和DNA电泳结果考察SAB是否对这些细胞有作用。结果QDs可清晰地示踪SAB与SPCA-1和7721细胞之间结合情况,通过常规方法首次证实了SAB对这两种细胞有增殖抑制作用。结论应用量子点纳米荧光探针直观发现中药有效成分与细胞之间的作用是可行的。

OBJECTIVE To study the interaction between the active components of Chinese traditional medicine and cells by Quantum Dots nanoprobes. METHODS Salvianolic acid B（SAB） was activated by 1-ethyl-3 （3-dimethylamino propyl ）-carbodiimide （ EDC ） and successfully conjugated with water-soluble quantum dots （ QDs ） using mercaptoethylamine （ ME ） as a crosslink reagent. The QDs-SAB bioconjugates obtained were then coclutured with lung cancer cells （ SPCA-1 ） and liver cancer cells （7721）. The toxici- ty of the SAB to these cells were studied by the observation of cell morphology, MTT and DNA gel electrophoresia. RESULTS The binding of SAB to the SPCA-1 and 7721 cells could be observed clearly by the fluorescence of QDs. According this result,we find that the SAB was toxic to the two cells by common cell experiments. CONCLUSION Using the QDs nanoprobes to find the interaction be- tween the active components of Chinese traditional medicine and cells is possible.