摘要
鼠科的骨胳的肌肉包含造血的干细胞，而是这个潜力到目前为止没被学习份量上或品质上试管内。确定造血的干细胞潜力，我们在 long-termculture 开始使用了高度净化的 SP/CD45 (+) 房间房间(LTC-IC ) 试金。从鼠科的肌肉净化的 SP/CD45 (+) 房间人口被发现从肌肉与 1/640 .Single-cell-sorted SP/CD45 (+) 房间的 LTC-IC 频率举办重要干细胞活动在白天 16 点的展出柔韧的 proliferative 活动试管内(380 褶层扩大) ，特别在有 OP-9 层的文化以后，那也支持胚胎的干细胞。放大房间人口与 myeloid 的证据从单个房间 exhibitedmultilineage 区别能力发源，淋巴并且 NK 房间标记。因此，我们的结果证明能被 LTC-IC 试金确定的造血的干细胞第一次也在鼠科的骨胳的肌肉和表演存在，在单个房间的水平，这些房间展出多行的年龄区别能力和主要 proliferative 潜力。
The murine skeletal muscle contains hematopoietic stem cells, but this potential has so far not been studied quantitatively or qualitatively in vitro. To quantify the hematopoietic stem cell potential, we have used highly purified SP/CD45^＋ cells in long-term culture initiating cell （LTC-IC） assays. The SP/CD45^＋ cell population purified from murine muscle was found to have significant stem cell activity with an LTC-IC frequency of 1/640. Single-cell-sorted SP/CD45^＋ cells from muscle exhibited robust proliferative activity in vitro at day 16 （380-fold amplification）, especially after culture with OP-9 layers that also support embryonic stem cells. Amplified cell populations originating from single cells exhibited multilineage differentiation ability with evidence of myeloid, lymphoid and NK cell markers. Thus, our results demonstrate that hematopoietic stem cells that can be quantified by LTC-IC assays exist in the murine skeletal muscle and show also for the first time, at the single-cell level, that these cells exhibit multilineage differentiation ability and major proliferative potential.
