单个鼻咽癌细胞的拉曼光谱分析的研究

Study of Raman Spectra of Single Carcinoma of Nasopharynx Cell

利用激光镊子拉曼光谱系统研究了鼻咽癌细胞株和正常人鼻咽部气道上皮细胞株的单个细胞的拉曼光谱,对于每个细胞在不同部位测3个点。结果显示:正常细胞和癌细胞的平均拉曼光谱有显著差异:正常的细胞光谱强度比癌细胞的明显要高;正常细胞的1304和1336 cm-1处峰的强度比值为1.05,癌细胞的为1.22。用PCA主成分分析和DFA判别分析分别对单个细胞的平均光谱和不同位置所取得的单独光谱进行分析,结果发现:PCA和DFA均可以把癌细胞和正常细胞正确区分,对于单独光谱,DFA的效果更好一些。同时还发现同一个细胞中不同的光谱位置对PCA和DFA的区分度影响不是很大;PCA和DFA的图中还表明癌细胞的均匀度要比正常细胞的差。以上的研究均表明:激光镊子拉曼光谱可以成为区别正常鼻咽细胞和鼻咽癌细胞的有效手段。

The Raman spectra from carcinoma of nasopharynx cell lines（CNE2） and normal airway epithelial cell lines（HBE） were investigated using a laser tweezers Raman spectroscopy（LTRS）.The Raman scattering measurements were obtained from three different places in every single cell.Visual inspection of the spectra shows that the differences observed in spectra of the cancer cells and normal cells are obvious.The peak ratio I1 304/I1 336 is 1.05 for the normal cell and 1.22 for the cancer cell.Using a combination of principal component analysis（PCA） and discriminant function analysis（DFA）,the authors are able to predict cancer cells,and normal cells and the DFA is better for single Raman spectrum.The sampling locations did not seriously affect the result of PCA and DFA. PCA and DFA also show that the uniformity of normal cells is better than that of cancer cells.The results indicate that the Raman spectra may offer the experimental basis for colorectal cancer diagnosis.