鲜红斑痣光动力治疗的荧光光谱监测

Monitoring of Photodynamic Therapy of Port Wine Stain by Fluorescence Spectroscopy

应用荧光光谱技术监测了鲜红斑痣(PWS)光动力治疗(PDT)中的光敏剂血药浓度与光产物生成。以532 nm倍频Nd∶YAG激光器作为PDT照射光源与荧光激发光源,以光谱仪与ICCD采集荧光光谱。在系统验证实验中,构建含有血卟啉单甲醚(HMME)的小鼠正常皮肤的荧光基本光谱,通过最小二乘光谱拟合区分HMME荧光(624 nm)与光产物荧光(652 nm)。含有PSD-007的病人患区荧光光谱拟合采用相同基本光谱,获得不同病人个体具有显著差异的光敏剂血药浓度曲线与光产物生成漂白曲线。所建立的荧光光谱监测系统与光谱拟合方法可为PDT精确量化剂量学方法的建立提供技术手段,所得结果有利于制定个性化的PDT治疗方案。

The blood drug level and the formation of photoproduct were monitored during photodynamic therapy（PDT） of port wine stain（PWS） by fluorescence spectroscopy.The irradiation was implemented by a 532 nm double-frequency Nd∶YAG laser,and the collection of fluorescence spectra was completed with the use of spectrograph and ICCD.In the experiment for validation of the system,the fluorescence basis spectra of hematoporphyrin monomethyl ether（HMME）-sensitized mouse normal skin were constructed,and,by least-square fitting,HMME fluorescence（624 nm） could be discriminated from that of photoproduct（652 nm）.The fitting of fluorescence spectra measured from PWS patients skin containing PSD-007 was performed with the same basis spectra as those from the mouse skin.The curves of blood drug level of different patients with significant variance,as well as those of formation and bleaching of photoproduct were obtained.Fluorescence spectroscopy monitoring system and fitting method presented here can provide technical means for rigorous quantitative PDT dosimetry method,and the results obtained here will make for the individual scheme of PDT.