微波加热自组装成环荧光显微成像技术在环境水样中的应用研究

Fluorescence Microscopic Study of Environmental Water Sample by a Self-Ordered Ring Technique with Microwave Heating

提出了一种基于溶剂毛细流效应的微波加热自组装成环荧光显微成像技术在环境水样中的应用方法。在pH 4.58的HAc-NaAc反应介质和聚乙烯醇-124存在下,盐酸小檗碱液滴在疏水性玻璃表面上受热挥发形成直径为1.1 mm,环带宽为19.2μm自组装环（self-ordered ring,SOR）,其荧光被苦味酸猝灭,猝灭强度与苦味酸的浓度成正比。当点样体积为0.1μL时,线性范围为1.3-30.0×10^-7mol·L^-1,方法的检测限为1.3×10^-8mol·L^-1。应用于天然水样及合成水样分析,回收率为96.3%-108.0%,RSD小于3.3%。该方法灵敏度比斑点分析提高50倍,比液相分析方法提高了60倍,且来源于背景的干扰大大降低。检测方法在环境、生化等领域具有广泛的实用性和优越性。

A self-ordered ring（SOR） technique with microwave heating is proposed for the environmental water samples based on the capillary flow of solvent on the solid surface of glass slide support.In the reaction medium of pH 4.58 HAc-NaAc containing poly（vinyl alcohol）-124,a SOR of berberine with the outer diameter of 1.1 mm and the ring belt width of 19.2 μm can be formed using microwave heating and its fluorescence was quenched by picric acid.The maximum fluorescence intensity（Imax） was found to be proportional to picric acid content.When a 0.1 μL droplet of berberine-picric acid mixture was spotted on the solid surface,picric acid in the range of 1.3-30.0×10^-7 mol·L^-1 can be detected,and the limit of detection can reach 1.3×10^-8 mol·L^-1.With the present method,the contents of picric acid in natural water samples and synthetic samples were satisfactorily determined with its recoveries of 96.3%-108.0% and RSD lower than 3.3%.The sensitivity enhanced 50 times than that by spot analysis,and 60 times than that in solution.Moreover,the interference from background was reduced greatly.The authors believe that the SOR technique will find wide practicability and superiority in both environment and biochemistry.