芨芨草愈伤组织器官发生及植株再生

In vitro Regeneration of Achnatherum splendens (Trin.) Nevski

芨芨草(Achnatherum splendens (Trin.) Nevski)种子消毒并在MS培养基上萌发获得无菌苗,以幼苗的叶鞘和胚轴为外植体诱导愈伤组织,经继代后进一步诱导不定芽及生根。研究结果表明,诱导愈伤组织最适合的培养基为B5+1.5mg.L-12,4-D+0.5mg.L-1NAA;诱导芽分化较适合的培养基为B5+0.5mg.L-16-BA+0.2mg.L-1NAA;1/4B5+1.0mg.L-1NAA+0.2mg.L-1IBA+1.0g.L-1活性炭培养基则有利于芨芨草试管苗的生根。本实验建立了完整的芨芨草植株再生体系,移栽成活率高。

We studied the regeneration of Achnatherum splendens （Trin.） Nevski in vitro and selected several favorable media. Germ-free plants were produced with germination on MS medium. Callus was induced with B5＋1.5 mg·L^-1 2,4-D＋0.5 mg·L^-1 NAA, with leaf sheath and hypocotyls as explants. After two subcultures, shoots and roots were induced. The medium containing B5＋ 0.5 mg·L^-16-BA ＋ 0.2 mg·L^-1NAA was suitable for shoot induction. For root induction, the medium containing 1/4 B5＋1.0 mg·L^-1 NAA＋ 0.2 mg·L^-1 IBA ＋1.0 g·L^-1 active carbon was favorable. With this regeneration system for A. splendens （Trin.） Nevski, we achieved the highest survival rate of transplants.