微分化急性髓系白血病患者细胞化学特点分析(附2例报告)

Brief analysis of cytochemistry characteristics of minimally differentiated acute myeloid leukemia patient's——two reports attached

目的:探讨微分化急性髓系白血病(AML-M0)化学特点并与其他类型白血相鉴别。方法:常规细胞化学染色方法。结果:M0的原始细胞过氧化物酶(POX)、苏丹黑(SBB)、特异性酯酶(CE)染色均为阴性,而酸性非特异性酯酶(ANAE)及糖原(PAS)染色阳性率较高,ANAE酶型与M1、急性淋巴细胞白血病(ALL)相似,表现为局灶型且不被氟化钠(NaF)抑制,PAS酶型为细颗粒弥散状分布,在此基础上夹有珠状、块状,而ALL酶型为细颗粒、中粗颗粒和粗颗粒散在分布,部分细胞可见珠状、块状且背景较清晰干净。结论:ANAE、PAS在细胞化学染色中对AML-M0的诊断有很重要的参考价值,与细胞形态学、免疫表型3者相结合,能极大地提高AML-M0的诊断率。

Objective：To discuss the characterisics of minmally differentiated acute myeloid leukemia and disinguish it with other types of leukemia. Method： Routine cytochemical staining methods were performed. Result： POX, SBB, CE staining were negative in myeloblasts from M0 patients, while AE and PAS staining were relatively high. Similar like in AML-M1 and ALL, AE enzyme in AML-M0 was focal type and could not be inhibited by NaF. PAS enzyme in M0 was fine-granular and mainly diffused but with few beads or clumps. Otherwise PAS enzyme in ALL was diffused fine-granular,medium-size-granular and coarse-granular, bead-type or clumps-type only could be detected in few ceils and the background was clear and clean. Conelusion：ANAE, PAS staining is important for diagnosis of AML-M0. It helps increase diagnosis rate while combined with cytomophology and immunophenootype.