骨髓增生异常综合征骨髓克隆造血细胞高表达WT1

WT1 is predominantly expressed in clonal hematopoietic cells from bone marrow of myelodysplastic syndromes

目的:调查骨髓增生异常综合征(MDS)骨髓Wilms'肿瘤基因(WT1)是否高表达于恶性克隆细胞。方法:对18例具有克隆标记MDS的骨髓细胞进行了WT1免疫细胞化学(APAAP,碱性磷酸酶-抗碱性磷酸酶)和DNA荧光原位杂交(FISH)的共检测。并与12例正常献血员进行对照。结果:MDS骨髓细胞明显高表达WT1,与对照比较P<0.05。双重染色的结果显示WT1突出地表达于具有异常染色体的恶性克隆造血细胞,而不是正常杂交信号的残存正常造血细胞(P<0.05)。结论:推测MDS骨髓WT1高表达与细胞增殖状态相关。

Objective： To determine the expression of Wilms＇tumor gene （WT1） in clonal hematopoietic cells form bone marrow of patients with myelodysplastic syndromes （MDS）. Method： Immunochemistry labeling （APAAP, alkaline phosphatase anti-alkaline phosphatase） and FISH （Fluorescence in situ hybridization） were coperformed on bone marrow cytospins from 18 patients whose abnormal karyotypes had been determined by G-binding analysis. Samples from 12 healthy donors were taken as controls. Result：Compared to 12 healthy donors, WT1 positive nucleated cells in MDS marrows were significantly higher （ P %0.05）. Moreover, WT1 was expressed predominantly in MDS clonal cells （with abnormal FISH signals） rather than in non-clonal cells （residual normal cells） （ P〈0.05）. Conclusion： It was proposed that high-expression of WT1 in MDS marrow perhaps just presen ted active cells proliferation.