TFF2在幽门螺杆菌感染胃体部黏膜中的表达

Expression of trefoil factor family 2 in fundic glands with Helicobacter pylori infection

目的:探讨三叶因子2(TFF2)在幽门螺杆菌(H pylori)感染胃体腺黏膜中表达的意义.方法:选择有H pylori感染的胃手术切除标本29例(92个蜡块),其中胃及十二指肠溃疡5例,胃癌24例,用免疫组织化学方法检测TFF2的分布和表达.结果:在H pylori感染胃黏膜中,TFF2主要在胃体腺的颈黏液细胞和假幽门腺化生细胞中表达;在正常黏膜中TFF2主要分布在胃固有腺的上1/3-2/3;在轻度萎缩黏膜中,腺底部仅见少量阳性细胞,随着萎缩程度的加重,在腺底部出现的阳性细胞增多;在轻、中度萎缩黏膜中其强阳性率分别为18.0%和42.9%,而在重度萎缩中达90.5%.在轻、中度和重度萎缩之间有显著性差异(P<0.01).结论:H pylori感染增加在腺底部的TFF2表达,并与黏膜的萎缩程度有关.

AIM： To investigate the protein expression of the trefoil factor family 2 （TFF2） in fundic glands with Helicobacter pylori infection. METHODS： Protein expression of TFF2 was detected by immunohistochemistry in paraffinembedded samples from 29 （92 blocks） surgical specimens from five patients gastric ulcer and 24 with gastric carcinoma. RESULTS： Expression of TFF2 was found in mucous neck cells and cells with pseudopylori gland metaplasia. Positive cells were distributed above the 1/2 to 2/3 portions of fundic glands in normal gastric mucosa. The ratio of TFF2- positive cells in the basal portion of the fundic glands was 18.0, 42.9 and 90.5% in mild, moderate and severe glandular atrophy, respectively, and these differences were significant （P 〈 0.01）. CONCLUSION： H pylori infection increases the expression of TFF2 in deeper parts of the fundic gland, and is also associated with the degree of glandular atrophy. The expression of TFF2 is related to mucosal protection and repair processes.