龈沟冲洗液的蛋白质含量测定

Determination of Protein in Gingival Crevicular Fluid Obtained by Intracrevicular Washing Method

本研究采用龈沟冲洗法检测牙周健康部位和疾病部位的龈沟液蛋白质含量,旨在分析蛋白含量与牙周病变的关系.结果表明牙周炎组的龈沟回吸液量及总蛋白含量显著高于健康、龈沟炎组,健康与龈炎组的回吸液量几乎无差别.龈沟回吸液量和蛋白含量均与临床指标一出血指数、菌斑指数、探诊深度、附着丧失显著相关,其中与探诊深度的相关性较大,提示龈沟冲洗法所得冲洗液量受牙周袋深度的影响.回吸液中蛋白含量随着临床炎症加重而升高,说明回吸液中总蛋白含量的高低反映了炎症的程度.

This study examined the amout of protein of the gingival crevicular fluid obtained from' healthy sites and diseased sites by an intracrevicular washing method. The washing solution volume and the total amount of protein from periodontitis sites were statistically higher than those from gingivitis sites and healthy sites. The washing solution volume and the amount of protein statistically correlated with Bleeding index, Plaque index, Pocket depth and Attatchment loss. The significant correlation with PD and BI suggested that the pocket depth had an effect on the volume of the washing solution. The protein amount of the washing solution increased with the aggravated inflammation. It suggested that the protein amount of the washing solution reflected the degree of inflammation to some extent.