云南省无偿献血者弓形虫感染状况

Study on Toxoplasma Gondii Infection in Blood Donors of Yunnan Province

目的了解云南省无偿献血者弓形虫感染情况,为制定科学的防治措施提供依据。方法采用酶联免疫吸附试验(ELISA)检测献血者血清弓形虫IgM和IgG抗体,对血清学阳性和随机选取的200份血清学阴性血样进行荧光定量PCR(FQ-PCR)检测,并对两种方法的检测结果进行相关性分析。结果云南省无偿献血者弓形虫IgM、IgG阳性率分别为0.47%和20.15%,IgM与FQ-PCR阳性结果的相关性有显著性,IgG与FQ-PCR阳性结果的相关性无显著性。结论云南省无偿献血者弓形虫IgG阳性率较国内其他地区高,考虑成本因素,可采用检测弓形虫IgM的方法进行献血者筛查。

Objective To understand the status of toxoplasma gondii （TOX） infection in Yunnan province blood donors,to provide basis for making practical control measures. Methods IgM and IgG antibodies against Toxoplasma in blood donors＇ sera were tested by enzyme-linked immunosorbent assay （ELISA）, then all seropositive and 200 random seronegative samples were detected by fluorescent quantitative PCR （FQ-PCR）. We analyzed the correlation between these two methods. Results The positive rate of IgM and IgG antibodies against the toxoplasma were 0. 47% and 20.15% respectively. The results of TOX-IgM and FQ-PCR-DNA were consistent. But for TOX-IgG they were not. Conclusion The rate of TOX-IgG of blood donor in Yunnan province is comparatively high. TOX-IgM can substitute for FQ-PCR in blood screening of Toxoplasma infection in blood donors.