TPPA-凝胶结合法用于梅毒特异性抗体检测的实验研究

A New Method of Integrating TPPA with Gel System Used to Confirm the Special Antibody of Syphilis

目的研究建立梅毒特异性抗体快速检测的实验方法。方法选取献血者TP-ELISA检测阳性标本46份和RPR法检测阳性的临床梅毒就诊患者的标本14份,以TPPA法为梅毒特异性抗体检测的对照,探讨TPPA与微凝胶柱法相结合的方法检测梅毒特异抗体的可行性。首先在96微孔板中对待检标本进行一定的稀释,然后按TPPA法的要求在相应的稀释孔中加入融化完全的D阴性颗粒、C梅毒抗原包被的阳性颗粒,混匀后注入微凝胶柱上腔中37℃孵育、离心,判读结果。结果46份献血者的标本中,TPPA-凝胶结合法与TPPA法均为阳性的有36份,均为阴性的10份;14份临床梅毒就诊患者的标本,两法均为阳性的13份、阴性1份。结合法与TPPA法的实验结果完全一致,符合率为100%。TPPA-凝胶结合法比TPPA法实验的观测时间大大地缩短,从2h缩短为30min,整个检测时间<1h。结论TPPA-凝胶结合法用时短,结果易于判读和保存,可用于梅毒特异性抗体的快速检测。

Objective To eastablish a rapid assay method used to confirm the antibody of Syphilis. Method To estabilish a combined method of TPPA and the gel system. TPPA was acted as a essential and comparative method. There were 46 TP-ELISA positive examples from donors and 14 RPR positive examples from the patients of Syphili. First diluting the examples in the 96 microwell plate,then adding the D negative- and C positive-partical in the different wells,taking these mixed liquid into the upper of the reaction chamber of the gel. The gel system was incubated at 37℃ and then centrifuged. At last the results were examined. Results Among 46 examples of the donors there were 10 negative ; and 14 examples of the patients,1 negative. The results of the TPPA and the new method were identical. The rate of uniform was 100%. Conclusion The new method is special,quick method and greatly shorten the time observed,only 30 minute. The result can be conserved easily. The new method is suitable to assay the antibody of Syphilis.